3LMV
D-Tyr-tRNA(Tyr) Deacylase from plasmodium falciparum in complex with hepes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-10-29 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 51.650, 52.950, 89.680 |
Unit cell angles | 75.40, 74.20, 86.10 |
Refinement procedure
Resolution | 29.243 - 2.833 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lmt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.038 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.920 |
High resolution limit [Å] | 2.820 | 2.820 |
Rmerge | 0.062 | 0.313 |
Number of reflections | 20391 | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 95.8 | 79.5 |
Redundancy | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M TRIS/HEPES, 25-30% PEG3350, pH 7.5-8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |