3LMT
Crystal structure of DTD from Plasmodium falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-10-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 55.340, 57.880, 91.630 |
Unit cell angles | 102.80, 105.90, 99.60 |
Refinement procedure
Resolution | 29.668 - 2.750 |
R-factor | 0.184 |
Rwork | 0.181 |
R-free | 0.23500 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.025 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.840 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.095 | 0.364 |
Number of reflections | 26067 | |
<I/σ(I)> | 13.5 | |
Completeness [%] | 96.8 | 89.1 |
Redundancy | 8 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350, 0.1M MES, PH 6.2-6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |