3LK3
Crystal structure of CapZ bound to the CPI and CSI uncapping motifs from CARMIL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2008-11-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.124, 115.535, 61.675 |
| Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.680 |
| R-factor | 0.1601 |
| Rwork | 0.155 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lk2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.140 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.270 | 2.800 |
| High resolution limit [Å] | 2.680 | 2.700 |
| Rmerge | 0.049 | 0.140 |
| Number of reflections | 17727 | |
| <I/σ(I)> | 29.7 | 8.9 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2M KCl, 20% (w/v) PEG 3350 , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
