3LHP
Crystal structure of HIV epitope-scaffold 4E10_D0_1ISEA_004_N 4E10 Fv complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 107 |
Detector technology | CCD |
Collection date | 2008-03-10 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.977, 88.623, 149.997 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.990 - 2.700 |
R-factor | 0.24732 |
Rwork | 0.243 |
R-free | 0.33503 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Computationally-derived model of the epitope-scaffold Fv complex with the Fv based on PDB ID 1TZG. |
RMSD bond length | 0.009 |
RMSD bond angle | 1.132 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.102 | 0.405 |
Number of reflections | 19356 | |
<I/σ(I)> | 7.1 | 2.2 |
Completeness [%] | 96.2 | 85.5 |
Redundancy | 3.45 | 2.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 2000 MME, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |