3LH5
Crystal Structure of the SH3-Guanylate kinase core domain of ZO-1
Experimental procedure
| Experimental method | MIRAS |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 64 |
| Unit cell lengths | 125.800, 125.800, 35.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.240 - 2.600 |
| R-factor | 0.21288 |
| Rwork | 0.205 |
| R-free | 0.28545 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.279 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.650 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 10199 | |
| <I/σ(I)> | 15.5 | 1.9 |
| Completeness [%] | 99.7 | 58.8 |
| Redundancy | 8.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 M Hepes pH 7.5, 7% isopropanol, 15-20% PEG3350, 1-5 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
