3LGL
Crystal structure of the 53BP1 tandem tudor domain in complex with p53K382me2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-11 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.430, 78.070, 36.190 |
| Unit cell angles | 90.00, 121.96, 90.00 |
Refinement procedure
| Resolution | 24.133 - 1.600 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g3r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.926 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.030 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.053 | 0.143 |
| Number of reflections | 59066 | |
| <I/σ(I)> | 14.3 | 6 |
| Completeness [%] | 95.4 | 94.4 |
| Redundancy | 3.53 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M HEPES-Na pH 7.0, 2% PEG 400 and 2.4 M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
