3LGD
Crystal structure of human adenosine deaminase growth factor, adenosine deaminase type 2 (ADA2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.90 |
Spacegroup name | P 1 |
Unit cell lengths | 63.212, 73.099, 80.662 |
Unit cell angles | 113.38, 94.18, 92.16 |
Refinement procedure
Resolution | 39.680 - 2.000 |
R-factor | 0.18705 |
Rwork | 0.185 |
R-free | 0.23397 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.379 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | 0.430 |
Number of reflections | 101215 | |
<I/σ(I)> | 8.8 | 1.9 |
Completeness [%] | 97.4 | 96.9 |
Redundancy | 2.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.04 | 293 | 40% MPD, 5% PEG 8000, 0.1M cacodylate, pH 6.04, VAPOR DIFFUSION, HANGING DROP, temperature 293K |