3LD8

Structure of JMJD6 and Fab Fragments

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	138.281, 138.281, 183.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.890 - 2.700
R-factor	0.233
Rwork	0.233
R-free	0.26000
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.610
Number of reflections	40942
Completeness [%]	75.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.1M Bis(2-hydroxyethyl)-amino-tris(hydroxymethyl)-methane, 2.1M Ammonium Sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.1M Bis(2-hydroxyethyl)-amino-tris(hydroxymethyl)-methane, 2.1M Ammonium Sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K