3LAF

Structure of DCC, a netrin-1 receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0720
Spacegroup name	P 65
Unit cell lengths	109.380, 109.380, 129.442
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.216
Rwork	0.211
R-free	0.23100
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.082	0.890
Number of reflections	34427
<I/σ(I)>	8.7
Completeness [%]	100.0	100
Redundancy	11.4	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	1.0 M Ammonium Sulfate, 0.1 M Bis-tris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	1.0 M Ammonium Sulfate, 0.1 M Bis-tris pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K