3L9E
Crystal structures of holo and Cu-deficient Cu/ZnSOD from the silkworm Bombyx mori and the implications in Amyotrophic lateral sclerosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-08 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 |
| Unit cell lengths | 118.470, 118.470, 81.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.000 - 2.050 |
| R-factor | 0.20347 |
| Rwork | 0.201 |
| R-free | 0.24788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1azv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.072 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.990 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.103 | 0.470 |
| Number of reflections | 41012 | |
| <I/σ(I)> | 11 | 2.8 |
| Completeness [%] | 97.8 | 87 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2M calcium acetate, 0.1M sodium cacodylate pH 6.5, 17% PEG 4000, 15% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 289 KK |
