3L8Y
Complex of Ras with cyclen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.27982 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 88.000, 88.000, 134.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.920 - 2.020 |
| R-factor | 0.19132 |
| Rwork | 0.189 |
| R-free | 0.23806 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.709 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.046 | 0.118 |
| Number of reflections | 12483 | |
| <I/σ(I)> | 24.39 | 10.85 |
| Completeness [%] | 97.8 | 97.9 |
| Redundancy | 4.16 | 4.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 17% PEG6000, 200mM CaCl2, 30mM TRIS/HCL, 5mM MgCl2, 2mM DTE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
