3L8K
Crystal structure of a dihydrolipoyl dehydrogenase from Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97958 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 114.186, 126.737, 165.993 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.624 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.027 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.121 | 0.669 |
| Number of reflections | 41969 | |
| <I/σ(I)> | 11.1 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 294 | 100mM MES pH 6.3, 22% PEG 6K, vapor diffusion, temperature 294K |
