3L8J
Crystal structure of CCM3, a cerebral cavernous malformation protein critical for vascular integrity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9551 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 77.445, 77.445, 108.629 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.050 |
| R-factor | 0.27895 |
| Rwork | 0.278 |
| R-free | 0.29260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THE P212121 MODEL PDB entry 3L8I |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 6699 | |
| <I/σ(I)> | 19.3 | 2.7 |
| Redundancy | 13.7 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.16 M calcium acetate, 0.08 M sodium cacodylate pH 6.5, 20% glycerol, 13.5% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
