3L82
X-ray Crystal structure of TRF1 and Fbx4 complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-03-23 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9792, 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.097, 68.097, 234.421 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
Rwork | 0.237 |
R-free | 0.26340 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.285 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.081 |
Number of reflections | 21197 |
<I/σ(I)> | 54.3 |
Completeness [%] | 96.2 |
Redundancy | 11.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.2 | 289 | 300 mM NH4H2PO4, 100 mM sodium citrate pH 6.2, 10% n-propanol and 10 mM DTT, VAPOR DIFFUSION, temperature 289K |