3L82

X-ray Crystal structure of TRF1 and Fbx4 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-03-23
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9792, 0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	68.097, 68.097, 234.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.400
Rwork	0.237
R-free	0.26340
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.285
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Rmerge	0.081
Number of reflections	21197
<I/σ(I)>	54.3
Completeness [%]	96.2
Redundancy	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.2	289	300 mM NH4H2PO4, 100 mM sodium citrate pH 6.2, 10% n-propanol and 10 mM DTT, VAPOR DIFFUSION, temperature 289K