3L7Z
Crystal structure of the S. solfataricus archaeal exosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.122, 145.484, 97.245 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.410 |
| R-factor | 0.266 |
| Rwork | 0.263 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.621 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 2.410 | 6.460 | 3.000 |
| Rmerge | 0.160 | 0.074 | 0.474 |
| <I/σ(I)> | 5.3 | ||
| Completeness [%] | 90.4 | 98.7 | 72.8 |
| Redundancy | 2.9 | 4.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 200 mM Mg(Ac)2, and 25 mM (NH4)2SO4), pH 8.6, vapor diffusion, hanging drop, temperature 298K |
