3L7U
Crystal structure of human NM23-H1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 154.510, 154.510, 111.821 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.22448 |
| Rwork | 0.224 |
| R-free | 0.24193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ucn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.897 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 63.510 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 46177 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 6.5% PEG 8000, 0.1M Na/K Phosphate pH 6.2, 0.2 NaCl,0.2M NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
