3L6R
The structure of mammalian serine racemase: Evidence for conformational changes upon inhibitor binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.543, 84.212, 70.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.780 - 1.700 |
| Rwork | 0.168 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.064 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.780 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.094 | 0.280 |
| Number of reflections | 35305 | |
| <I/σ(I)> | 16.1 | 4.5 |
| Completeness [%] | 97.1 | 78.9 |
| Redundancy | 12.45 | 5.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | PEG 3350, 200mM sodium malonate, 50mM MnCl2, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
