3L4W

Crystal complex of N-terminal Human Maltase-Glucoamylase with miglitol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9175
Spacegroup name	P 21 21 21
Unit cell lengths	93.707, 107.854, 111.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.000
R-factor	0.19925
Rwork	0.198
R-free	0.23208
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.524
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.119	0.079	0.543
Number of reflections	76058
<I/σ(I)>	13.3
Completeness [%]	96.5	95.8	83.6
Redundancy	7.4	7.1	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	20% PEG 3350, 0.2M sodium sulfate, pH 6.5, vapor diffusion, hanging drop, temperature 293K