3L46

Crystal structure of the second BRCT domain of epithelial cell transforming 2 (ECT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	42.158, 60.630, 74.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.482
R-factor	0.182
Rwork	0.181
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cou
RMSD bond length	0.016
RMSD bond angle	1.551
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.510
High resolution limit [Å]	1.480	4.010	1.480
Rmerge	0.088	0.032	0.928
Number of reflections	32244
<I/σ(I)>	9.1
Completeness [%]	100.0	99.9	100
Redundancy	8.5	8.1	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	1.5M sodium citrate, 0.1M TRIS, 1:100 (w/w) subtilisin, pH 7.5, vapor diffusion, hanging drop, temperature 291K