3L43
Crystal structure of the dynamin 3 GTPase domain bound with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-21 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.286, 166.500, 74.985 |
| Unit cell angles | 90.00, 109.02, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.270 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aka |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.310 |
| High resolution limit [Å] | 2.270 | 6.110 | 2.270 |
| Rmerge | 0.068 | 0.025 | 0.583 |
| Number of reflections | 52532 | ||
| <I/σ(I)> | 14.6 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 3.6 | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% PEG3350, 0.2M sodium chloride, 0.1M HEPES. 1:100 w/w subtilisin., pH 7.5, vapor diffusion, sitting drop, temperature 291K |
