3L2R

Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2009-10-13
Detector	ADSC
Wavelength(s)	0.9796
Spacegroup name	P 41 21 2
Unit cell lengths	158.480, 158.480, 124.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.999 - 2.880
R-factor	0.20305
Rwork	0.202
R-free	0.22232
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3DLR; 1EX4 CHAIN A RESIDUES 223-266; 7bp B-DNA
RMSD bond length	0.013
RMSD bond angle	1.657
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.999	3.040
High resolution limit [Å]	2.880	2.880
Number of reflections	35972
Completeness [%]	99.2	100
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.35M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50mM Mes-NaOH, pH 6.5, vapor diffusion, hanging drop, temperature 291K