3L28
Crystal structure of Zaire Ebola VP35 interferon inhibitory domain K339A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.381, 91.880, 102.595 |
| Unit cell angles | 90.00, 99.60, 90.00 |
Refinement procedure
| Resolution | 33.770 - 2.400 |
| R-factor | 0.20352 |
| Rwork | 0.200 |
| R-free | 0.26908 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fke |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.873 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.044 | 0.433 |
| Number of reflections | 38190 | |
| <I/σ(I)> | 29 | 2.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 2.8 | 293 | 0.1M SODIUM CITRATE, 0.3M LITHIUM SULFATE, 13% PEG8000, pH 2.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
