3L1Z
Crystal structure of the U-BOX domain of human E4B ubiquitin ligase in complex with UBCH5C E2 ubiquitin conjugating enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-10 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 6 2 2 |
Unit cell lengths | 142.708, 142.708, 83.133 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.780 - 3.170 |
R-factor | 0.2364 |
Rwork | 0.232 |
R-free | 0.27940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1L1X AND 3L1Y |
RMSD bond length | 0.003 |
RMSD bond angle | 0.689 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.780 | 3.230 |
High resolution limit [Å] | 3.170 | 3.170 |
Rmerge | 0.192 | 0.780 |
Number of reflections | 8196 | |
<I/σ(I)> | 18.06 | 6.18 |
Completeness [%] | 92.2 | 96 |
Redundancy | 58.8 | 57.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 4M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |