3L0O
Structure of RNA-free Rho transcription termination factor from Thermotoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-17 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.931 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 139.000, 139.000, 150.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.550 - 2.350 |
R-factor | 0.26934 |
Rwork | 0.268 |
R-free | 0.29716 |
Structure solution method | SIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 2.004 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXS |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 81012 | |
Completeness [%] | 99.2 | 90.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 295 | 0.1M sodium acetate, 0.8M magnesium sulphate, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 295 | 0.1M sodium acetate, 0.8M magnesium sulphate, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |