3KYH
Saccharomyces cerevisiae Cet1-Ceg1 capping apparatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 63 |
| Unit cell lengths | 165.993, 165.993, 172.355 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.980 - 3.000 |
| R-factor | 0.2516 |
| Rwork | 0.249 |
| R-free | 0.29801 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.369 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.468 |
| Number of reflections | 52300 | |
| <I/σ(I)> | 12.7 | 1.9 |
| Completeness [%] | 97.7 | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 1.0 M ammonium citrate, 0.1 M sodium citrate, 1.0% PEG (polyethylene glycerol) 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
