3KVO
Crystal structure of the catalytic domain of human Hydroxysteroid dehydrogenase like 2 (HSDL2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-28 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97959 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.149, 78.332, 121.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.070 - 2.250 |
| R-factor | 0.20187 |
| Rwork | 0.199 |
| R-free | 0.24679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3E03.pdb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.442 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.070 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.110 | 0.895 |
| Number of reflections | 28262 | |
| <I/σ(I)> | 9.4 | 2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG 3350, 0.1M BIS-TRIS pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
