3KVO

Crystal structure of the catalytic domain of human Hydroxysteroid dehydrogenase like 2 (HSDL2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-28
Detector	RAYONIX MX-300
Wavelength(s)	0.97959
Spacegroup name	P 21 21 21
Unit cell lengths	61.149, 78.332, 121.765
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.070 - 2.250
R-factor	0.20187
Rwork	0.199
R-free	0.24679
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3E03.pdb
RMSD bond length	0.012
RMSD bond angle	1.442
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0089)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.070	2.370
High resolution limit [Å]	2.250	2.250
Rmerge	0.110	0.895
Number of reflections	28262
<I/σ(I)>	9.4	2
Completeness [%]	99.9	99.8
Redundancy	4.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	25% PEG 3350, 0.1M BIS-TRIS pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K