3KUT
Crystal structure of the MLLE domain of poly(A)-binding protein in complex with the binding region of Paip2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-30 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9950 |
Spacegroup name | P 1 |
Unit cell lengths | 26.309, 31.748, 48.454 |
Unit cell angles | 100.29, 90.37, 99.05 |
Refinement procedure
Resolution | 47.620 - 1.500 |
R-factor | 0.16917 |
Rwork | 0.168 |
R-free | 0.20065 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i2t |
RMSD bond length | 0.009 |
RMSD bond angle | 1.125 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 0.233 |
Number of reflections | 22328 | |
<I/σ(I)> | 20.8 | 3.4 |
Completeness [%] | 96.6 | 93.2 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 2.25M ammonium sulfate, 0.2M KBr, 0.1 M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |