3KTU
Structure of human 8-oxoGuanine Glycosylase 1 bound to fluorninated oxoG-containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 92.400, 92.400, 212.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.210 - 2.300 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ebm |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.112 | 0.397 |
| Number of reflections | 24522 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9.2 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 277 | Calcium Chloride, Sodium Cacodylate, pH 6.0, PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Calcium Chloride | ||
| 2 | 1 | 2 | Calcium Chloride | ||
| 3 | 1 | 1 | Sodium Cacodylate | ||
| 4 | 1 | 2 | Sodium Cacodylate | ||
| 5 | 1 | 1 | PEG 8000 | ||
| 6 | 1 | 2 | PEG 8000 |
