3KTP
Structural basis of GW182 recognition by poly(A)-binding protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9780 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 38.461, 38.461, 136.533 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.010 - 1.500 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.23067 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i2t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.005 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.056 | 0.294 |
Number of reflections | 16216 | |
<I/σ(I)> | 34.1 | 3.4 |
Completeness [%] | 98.5 | 82.7 |
Redundancy | 9.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 2.0 M ammonium sulfate, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |