3KS4
Crystal structure of Reston ebolavirus VP35 RNA binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 158.160, 158.160, 166.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.657 - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.21900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.4_151)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.660 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.544 |
| Number of reflections | 30094 | |
| <I/σ(I)> | 6.3 | 2 |
| Completeness [%] | 96.1 | 97.9 |
| Redundancy | 3.71 | 5.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295.5 | 90mM tripostassium citrate, 100mM sodium acetate, 5% (wt/vol) PEG 6000, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K |
