3KPB
Crystal Structure of the CBS domain pair of protein MJ0100 in complex with 5 -methylthioadenosine and S-adenosyl-L-methionine.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Collection date | 2009-09-19 |
Spacegroup name | P 1 |
Unit cell lengths | 46.987, 49.704, 59.120 |
Unit cell angles | 80.11, 75.85, 64.04 |
Refinement procedure
Resolution | 57.170 - 1.600 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.22900 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.144 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 57.170 |
High resolution limit [Å] | 1.600 |
Number of reflections | 55517 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 291 | 10% PEG 6000, 0.1 M TRIS pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |