3KOY

Crystal Structure of ornithine 4,5 aminomutase in complex with ornithine (Aerobic)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-07
Detector	ADSC QUANTUM 315
Wavelength(s)	1.072
Spacegroup name	P 1 21 1
Unit cell lengths	66.520, 233.220, 124.140
Unit cell angles	90.00, 103.43, 90.00

Refinement procedure

Resolution	60.373 - 2.800
R-factor	0.1874
Rwork	0.183
R-free	0.26250
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	1.418
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	120.750	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.096	0.444
<I/σ(I)>	7.7	2.6
Completeness [%]	93.8	87.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	A protein solution (8 mg/ml 4,5-OAM, 5 mM 2-mercaptoethanol, 10 mM Tris HCl pH 8.0, 2 mM PLP, and 2 mM AdoCbl) was mixed in a 1:1 ratio with precipitant solution [(0.1 M Tris HCl, pH 8.0, 0.2 M MgCl2, 25 % (wt/vol) polyethylene glycol 2000 mono-methylether] under red light at room temperature, VAPOR DIFFUSION, SITTING DROP, temperature 294K