3KOA
M296I mutant of foot-and-mouth disease virus RNA-polymerase in complex with a template- primer RNA and GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.371, 95.371, 100.878 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.890 - 2.400 |
| R-factor | 0.22607 |
| Rwork | 0.223 |
| R-free | 0.28985 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wne |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.210 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.910 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.096 | 0.880 |
| Number of reflections | 21201 | |
| <I/σ(I)> | 13.5 | 1.9 |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 5.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30% PEG 4000, 0.1M HEPES pH 7.0, 0.2M magnesium acetate, 4% butyrolactone, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
