3KNV
Crystal structure of the RING and first zinc finger domains of TRAF2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 43.669, 43.669, 284.393 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.800 - 1.900 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.23900 |
Structure solution method | SAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.830 | 4.970 | 1.830 |
Rmerge | 0.074 | 0.049 | 0.331 |
Number of reflections | 23319 | ||
<I/σ(I)> | 14.2 | ||
Completeness [%] | 99.0 | 97.7 | 84.7 |
Redundancy | 22.9 | 32 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% PEG 8000, 0.1 M HEPES pH 7.5, 8% Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |