3KMD

Crystal structure of the p53 core domain bound to a full consensus site as a self-assembled tetramer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Collection date	2009-02-07
Spacegroup name	P 21 21 21
Unit cell lengths	65.084, 93.713, 145.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.516 - 2.150
R-factor	0.2173
Rwork	0.216
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ac0
RMSD bond length	0.006
RMSD bond angle	1.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.138	0.085	0.614
Number of reflections	52544
<I/σ(I)>	6.8
Completeness [%]	99.9	100	98.7
Redundancy	7	6.9	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.68	291	16% PEG 4000, 142mM Nacl, pH 6.68, hanging drop, temperature 291K