3KM9

Structure of complement C5 in complex with the C-terminal beta-grasp domain of SSL7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-02-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.900
Spacegroup name	P 31
Unit cell lengths	144.790, 144.790, 245.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.752 - 4.200
R-factor	0.2364
Rwork	0.233
R-free	0.29710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3CU7 with the C345C domain removed
RMSD bond length	0.011
RMSD bond angle	1.551
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.400
High resolution limit [Å]	4.200	4.200
Number of reflections	41914
<I/σ(I)>	14.4
Completeness [%]	99.9	100
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.2	277	Reservoir contains 50mM MgAc2, 50mM MES pH 6.2. Mixed 1:1 with concentrated protein, VAPOR DIFFUSION, temperature 277K