3KLO
Vibrio cholerae VpsT bound to c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 121.703, 121.703, 208.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.862 - 2.802 |
| R-factor | 0.2483 |
| Rwork | 0.246 |
| R-free | 0.29040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kln |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 2009_02_15_2320_3)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 60780 | |
| <I/σ(I)> | 18.2 | 4 |
| Completeness [%] | 97.8 | 97.3 |
| Redundancy | 14.5 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M Tris-Cl, 0.8M potassium sodium tartrate tetrahydrate, 3-5% PEG-MME 5000, 8-12% Xylitol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
