3KH4
Crystal structure of human Cu/Zn superoxide dismutase recombinantly produced in Leishmania tarantolae; P6522 crystal form containing 6 chains in the asymmetric unit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 112.188, 112.188, 428.268 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.690 - 3.500 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.20800 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.434 |
Data scaling software | XSCALE |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.690 | 3.600 | |
High resolution limit [Å] | 3.500 | 20.000 | 3.500 |
Rmerge | 0.172 | 0.027 | 0.586 |
Number of reflections | 21168 | 148 | 1668 |
<I/σ(I)> | 13.13 | 43.1 | 4.2 |
Completeness [%] | 99.9 | 91.4 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 21-25% (w/v) PEG4000, 0.1 M NaOAc, pH 4.2-5.2, vapor diffusion, hanging drop, temperature 292K |