3KFU
Crystal structure of the transamidosome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9807 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 115.920, 214.000, 127.840 |
Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
Resolution | 39.189 - 3.000 |
R-factor | 0.2004 |
Rwork | 0.199 |
R-free | 0.25170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1N9W and 2F2A |
RMSD bond length | 0.007 |
RMSD bond angle | 0.734 |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 123945 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5 10% peg4000 0.2 M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |