3KDF
X-ray Crystal Structure of the Human Replication Protein A Complex from Wheat Germ Cell Free Expression
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9686, 0.97947 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.680, 77.700, 121.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.999 - 1.975 |
| R-factor | 0.192 |
| Rwork | 0.192 |
| R-free | 0.20600 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.352 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.101 | 0.566 |
| Number of reflections | 43894 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 97.9 | 82.7 |
| Redundancy | 13.6 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 11% PEG 8000, 50 mM BisTris-propane pH 7.0, 50 mM NaCl, 5 mM HEPES pH 7.0, 0.3 mM TCEP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
