3KDE
Crystal structure of the THAP domain from D. melanogaster P-element transposase in complex with its natural DNA binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.92000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.692, 69.325, 35.126 |
| Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
| Resolution | 35.090 - 1.740 |
| R-factor | 0.17935 |
| Rwork | 0.177 |
| R-free | 0.21580 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.323 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 26096 | |
| <I/σ(I)> | 21.6 | 2.6 |
| Completeness [%] | 95.0 | 66.8 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 24% PEG 8000, 5 mM NaCl, 50 mM CAPSO pH 9.0, 10 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | CAPSO | ||
| 4 | 1 | 1 | TCEP | ||
| 5 | 1 | 2 | PEG 8000 | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | CAPSO | ||
| 8 | 1 | 2 | TCEP |
