3KD6
Crystal Structure of Nucleoside Kinase from Chlorobium tepidum in Complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.014, 89.797, 64.827 |
| Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
| Resolution | 46.870 - 1.880 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 51078 | |
| <I/σ(I)> | 7.7 | 5.9 |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 7.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 0.2M SODIUM CHLORIDE 0.1M BIS-TRIS pH 5.5 20% PEG 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
