3KD6
Crystal Structure of Nucleoside Kinase from Chlorobium tepidum in Complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.014, 89.797, 64.827 |
Unit cell angles | 90.00, 92.90, 90.00 |
Refinement procedure
Resolution | 46.870 - 1.880 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.23700 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Number of reflections | 51078 | |
<I/σ(I)> | 7.7 | 5.9 |
Completeness [%] | 99.7 | 97.4 |
Redundancy | 7.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 0.2M SODIUM CHLORIDE 0.1M BIS-TRIS pH 5.5 20% PEG 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 292K |