3KA8
Frog M-ferritin, EQH mutant, with cobalt
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.1159 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 183.490, 183.490, 183.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.350 |
R-factor | 0.131 |
Rwork | 0.130 |
R-free | 0.15300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ka3 |
RMSD bond length | 0.024 |
RMSD bond angle | 1.957 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | PHASER |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.420 |
High resolution limit [Å] | 1.350 | 4.270 | 1.350 |
Rmerge | 0.085 | 0.031 | 0.835 |
Total number of observations | 27277 | 78942 | |
Number of reflections | 58309 | ||
<I/σ(I)> | 19.2 | 13.4 | 0.9 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 13.1 | 13 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 2M MgCl2, 0.1M Bicine pH 9.0, 0.1M CoCl2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |