3K75
X-ray crystal structure of reduced XRCC1 bound to DNA pol beta catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-30 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.770, 44.050, 152.770 |
| Unit cell angles | 90.00, 107.21, 90.00 |
Refinement procedure
| Resolution | 24.470 - 2.950 |
| R-factor | 0.245 |
| Rwork | 0.242 |
| R-free | 0.29500 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.061 | 0.249 |
| Number of reflections | 19123 | 1376 |
| <I/σ(I)> | 17.7 | 4.3 |
| Completeness [%] | 98.7 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20-25% PEG 3350, 0.2-0.3M Tri-potassium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
