3K70
Crystal structure of the complete initiation complex of RecBCD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.800, 192.900, 334.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.590 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w36 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.790 |
| High resolution limit [Å] | 3.590 | 3.590 |
| Rmerge | 0.075 | 0.351 |
| Number of reflections | 97332 | |
| <I/σ(I)> | 6.6 | 2.1 |
| Completeness [%] | 96.7 | 97.5 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 285 | 100 mM Hepes pH 7.0, 300 mM Calcium acetate, 6-8% PEG 20000, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Hepes | ||
| 2 | 1 | 1 | Calcium acetate | ||
| 3 | 1 | 1 | PEG 20000 | ||
| 4 | 1 | 2 | Hepes | ||
| 5 | 1 | 2 | Calcium acetate | ||
| 6 | 1 | 2 | PEG 20000 |
