3K5L

Crystal structure of E.coli Pol II-abasic DNA-dATP Lt(0, 3) ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2007-06-14
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	79.974, 101.198, 121.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.420 - 2.700
Rwork	0.224
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k57
RMSD bond length	0.205
RMSD bond angle	2.161
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.160	0.565
Number of reflections	24052
<I/σ(I)>	9	2.8
Completeness [%]	87.4	81.7
Redundancy	4.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEGMME3350, 0.2 M ammonium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K