3K5L
Crystal structure of E.coli Pol II-abasic DNA-dATP Lt(0, 3) ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.974, 101.198, 121.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.420 - 2.700 |
| Rwork | 0.224 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k57 |
| RMSD bond length | 0.205 |
| RMSD bond angle | 2.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.160 | 0.565 |
| Number of reflections | 24052 | |
| <I/σ(I)> | 9 | 2.8 |
| Completeness [%] | 87.4 | 81.7 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEGMME3350, 0.2 M ammonium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
