3K57
Crystal structure of E.coli Pol II-normal DNA-dATP ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2006-10-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.425, 99.888, 125.745 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.080 |
Rwork | 0.204 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q8i |
RMSD bond length | 0.010 |
RMSD bond angle | 1.487 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.070 | 0.478 |
Number of reflections | 57617 | |
<I/σ(I)> | 13.6 | 1.8 |
Completeness [%] | 93.5 | 87 |
Redundancy | 2.4 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES, 20% PEGmme5000, 0.2 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |