3K3R
Unrefined crystal structure of a LexA-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.357, 120.393, 149.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 14447 |
| <I/σ(I)> | 13.1 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 8.5 | 292 | 40mM Tris-HCl, 1mM EDTA, 10mM MgCl2, 0.1M NaCl, 5mM spermidine, 10% glycerol, 12% (w/v) PEG5000, pH 8.5, hanging drop, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris-HCl | ||
| 10 | 1 | 2 | MgCl2 | ||
| 11 | 1 | 2 | NaCl | ||
| 12 | 1 | 2 | spermidine | ||
| 13 | 1 | 2 | glycerol | ||
| 14 | 1 | 2 | PEG5000 | ||
| 2 | 1 | 1 | EDTA | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | NaCl | ||
| 5 | 1 | 1 | spermidine | ||
| 6 | 1 | 1 | glycerol | ||
| 7 | 1 | 1 | PEG5000 | ||
| 8 | 1 | 2 | Tris-HCl | ||
| 9 | 1 | 2 | EDTA |
