3K33
Crystal structure of the Phd-Doc complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 48.295, 48.295, 347.321 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.825 - 2.400 |
| R-factor | 0.2583 |
| Rwork | 0.257 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dd7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.266 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.085 | 0.224 |
| Number of reflections | 19121 | |
| <I/σ(I)> | 23.2 | 10.8 |
| Completeness [%] | 100.0 | 97.8 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100 mM Sodium acetate pH 4.5, 20 mM CaCl2 and 35-40% v/v MPD, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
