3K26

Complex structure of EED and trimethylated H3K4

Experimental procedure

Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.920, 85.310, 91.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 1.580
R-factor	0.164
Rwork	0.162
R-free	0.19659
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.031
RMSD bond angle	2.409
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.940
High resolution limit [Å]	1.580
Number of reflections	58074
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	3.5M NaF, 10 mM TCEP, 15%Glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K